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The molecular mechanism(s) by which curcumin affects sperm motility has not been delineated. The Effect of Curcumin on Intracellular pH ( pHi), Membrane Hyperpolarization and Sperm Motility.Ĭurcumin has shown to affect sperm motility and function in vitro and fertility in vivo. Our results suggested that the declined Na(+)/K(+)-ATPase activity at acidic pHs result in decreased sperm movement and capacitation, which could be one of the mechanisms of male infertility. Compared with that at pH 7.2, the mean fluorescence intensity of sperm in pH 5.2 and 6.2 media decreased significantly, while that of pH 8.2 group showed no difference.
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Using flow cytometry (FCM) and laser confocal scanning microscopy (LCSM) analysis, the intracellular Ca2(+ )concentrations of sperm cultured in sperm capacitation solution at pH 5.2, 6.2, 7.2 and 8.2 were determined. In addition, the sperm Na(+)/K(+)-ATPase activity at different pHs was measured, and the enzyme activity was significantly lower in pH 5.2 and 6.2 media, comparing with that in pH 8.2 and pH 7.2 solutions.
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The HOS rate exhibited positive correlation with the sperm total motility and PR. Our results showed that these parameters were similar in pH 7.2 and 8.2 sperm nutrition solutions, but decreased in pH 5.2 and 6.2 solutions. The spermatozoa cultured in sperm nutrition solution at pH 5.2, 6.2, 7.2 and 8.2 were analyzed for sperm total motility, progressive motility (PR), hypo-osmotic swelling (HOS) rate, and sperm penetration. The sperm in this study was collected from healthy males to avoid interference from other factors. Zhou, Ji Chen, Li Li, Jie Li, Hongjun Hong, Zhiwei Xie, Min Chen, Shengrong Yao, BingĪs the chemical environment of semen can have a profound effect on sperm quality, we examined the effect of pH on the motility, viability and capacitation of human sperm. The Semen pH Affects Sperm Motility and Capacitation.